Protein_Domain
Intein

Part:BBa_K300004

Designed by: Sara Bisi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-03)

Engineered pH-inducible intein (codon optimized for E. coli) - internal domain

Naturally occurring inteins are protein segments that are able to excise themselves from a precursor protein and rejoin the remaining portions (the exteins) with a peptide bond.


This part is a highly engineered intein that is able to self-cleave its C-terminus at low pH. For this reason, protein domains can be easily cleaved by using this intein without the need of proteases and their cleaving sites.


The optimal pH for cleavage has been reported to be 6.0, while a pH of 8.5 has been shown to inhibit the cleavage [see reference 1].


C-terminus cleavage is also strongly affected by temperature >20°C [see reference 1 for details].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 460


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Parameters
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